This paper reports bacterial cellulose composites made by blending chitosan, poly(ethylene glycol) (PEG), and gelatin for potential biomedical application of tissue-engineering scaffold and wound-dressing material. The bacterial cellulose composites were successfully prepared by immersing a wet bacterial cellulose pellicle into chitosan, PEG, or gelatin solutions followed by freeze-drying. The products look like a foam structure. Scanning electron microscopy images show that chitosan molecules penetrated into bacterial cellulose forming a multilayer and a well interconnected porous network structure with a large aspect surface. The morphology of the bacterial cellulose/gelatin scaffold indicates that the gelatin molecules could penetrate well between the individual nanofibers of the bacterial cellulose. Cell adhesion studies for these composites were carried out using 3T3 fibroblast cells. They showed much better biocompatibility than pure bacterial cellulose. Preparation and material characterization of these composites are explained.