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Research Paper

Holographically Fabricated Dye-Doped Nanoporous Polymers as Matrix for Laser Desorption/Ionization Mass Spectrometry

[+] Author and Article Information
Vincent K. S. Hsiao

Department of Applied Materials and Optoelectronic Engineering, National Chi Nan University, Nantou, 54561 Taiwan; Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802kshsiao@ncnu.edu.tw

Yue Bing Zheng

Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802yzz113@gmail.com

Heike Betz

The Huck Institutes of the Life Science, The Pennsylvania State University, University Park, PA 16802hsb6@psu.edu

Brian Kiraly

Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802btk5051@psu.edu

Wei Yan

Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802wyan.nc@gmail.com

Pamela F. Lloyd

Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH 45433pamela.lloyd@wpafb.af.mil

Timothy J. Bunning

Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH 45433timothy.bunning@wpafb.af.mil

Alexander N. Cartwright

Institute for Lasers, Photonics and Biophotonics, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000anc@buffalo.edu

Tony Jun Huang

Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802junhuang@psu.edu

J. Nanotechnol. Eng. Med 1(4), 041011 (Oct 29, 2010) (5 pages) doi:10.1115/1.4002610 History: Received September 08, 2010; Revised September 21, 2010; Published October 29, 2010; Online October 29, 2010

We report laser desorption/ionization mass spectrometry using a dye-doped nanoporous polymer matrix. The nanoporous polymer matrix was fabricated through a holographic interference patterning technique. The periodically aligned nanopores in the resulting polymer matrix produced a high surface-to-volume ratio that facilitates the homogeneous cocrystallization of the matrix and an analyte (i.e., peptide in this demonstration). To generate nanostructures with further enhanced functionalities, dyes were also incorporated into the photopolymer. We demonstrate that by using the dye-doped nanoporous polymer matrix, we can identify peptides with an enhanced signal from the peptides and decreased noise from the ion fragmentation. These results indicate that the dye-doped nanoporous polymer matrix we use here can be a promising platform for laser desorption/ionization mass spectrometry.

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Copyright © 2010 by American Society of Mechanical Engineers
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Figures

Grahic Jump Location
Figure 2

Surface morphology of the nanoporous polymer functionalized with C456 and trifluoroacetic acid, characterized by SEM

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Figure 3

(a) Experimental setup for dye-doped nanoporous polymer-assisted LDI spectrometry. (b) Cross-sectional morphology of dye-doped nanoporous polymer.

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Figure 4

Mass spectra of [Glu1]-fibrinopeptide B human (MW 1570.57) obtained by using (a) nanoporous polymer film doped with C456, (b) nanoporous polymer film doped with C456 and trifluoroacetic acid, and (c) nonporous polymer film doped with C456 and trifluoroacetic acid. The inset shows the enlarged high mass region from 1400 to 1800 m/z.

Grahic Jump Location
Figure 5

Mass spectra of control samples. (a) Mass spectra of peptide using nanoporous polymer film that does not contain C456. (b) Mass spectra of peptide using C456-doped nanoporous polymer film that does not contain trifluoroacetic acid.

Grahic Jump Location
Figure 6

Surface morphology of the nanoporous polymer film doped with C456 and trifluoroacetic acid (a) before and (b) after adding peptide onto the top of the film

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Figure 1

Schematic of the optical setup for fabricating one-dimensional, nanoporous polymer

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