0
Research Papers

Effects of Dissimilar Electrode Materials and Electrode Position on DNA Motion During Electrophoresis

[+] Author and Article Information
Regis A. David, Larry L. Howell

Department of Mechanical Engineering, Brigham Young University, Provo, UT 84602

Brian D. Jensen1

Department of Mechanical Engineering, Brigham Young University, Provo, UT 84602bdjensen@byu.edu

Justin L. Black, Sandra H. Burnett

Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602

1

Corresponding author.

J. Nanotechnol. Eng. Med 2(2), 021014 (May 17, 2011) (6 pages) doi:10.1115/1.4003600 History: Received January 07, 2011; Revised January 27, 2011; Published May 17, 2011; Online May 17, 2011

Electrophoretic systems commonly use metal electrodes in their construction. This paper explores and reports the differences in the electrophoretic motion of DNA (decomposition voltage, electrical field, etc.) when one electrode is constructed from a semiconductor, silicon, rather than metal. Experimental VI (voltage-current) curves for different electrode configurations (using steel and silicon) are presented. Experimental results are used to update and validate the mathematical model to reflect the differences in material selection. In addition, the model predicts large curved-field motion for DNA motion. The model helps to quantify the effect of parameters on DNA motion in biological microelectromechanical systems in order to improve device designs and protocols.

FIGURES IN THIS ARTICLE
<>
Copyright © 2011 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.

References

Figures

Grahic Jump Location
Figure 5

Decomposition voltage: effects of voltage on current in 120 mg agarose/40 ml TAE

Grahic Jump Location
Figure 6

DNA ladder experiment (St(−)St(+) in 9×7.5 cm2 box, 40 min at 60 V)

Grahic Jump Location
Figure 7

Comparison of DNA trajectory lines (St(−)St(+) in 9×7.5 cm2 box, 60 min at 10 V)

Grahic Jump Location
Figure 8

St(−)St(−)St(+) electrodes in a 15.8×14 cm2 box for 120 min at 10 V

Grahic Jump Location
Figure 9

Comparison of DNA trajectory lines, 120 min at 5 V

Grahic Jump Location
Figure 10

Linear dependence between modeled and experimental results from Table 2

Grahic Jump Location
Figure 3

A schematic representation of experimental setup (dimensions are not to scale)

Grahic Jump Location
Figure 4

A schematic representation for the applied potential between two electrodes

Grahic Jump Location
Figure 1

Image of the MEMS lance assembly (10×)

Grahic Jump Location
Figure 2

Ohm’s law in electrolyte conductors (V0 is the decomposition voltage)

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In