0
Research Paper

Preparation and Characterization of Novel Bacterial Cellulose/Gelatin Scaffold for Tissue Regeneration Using Bacterial Cellulose Hydrogel

[+] Author and Article Information
Zhijiang Cai

Department of Mechanical Engineering, Center for EAPap Actuator, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Korea

Jaehwan Kim

Department of Mechanical Engineering, Center for EAPap Actuator, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Koreajaehwan@inha.ac.kr

J. Nanotechnol. Eng. Med 1(2), 021002 (May 05, 2010) (6 pages) doi:10.1115/1.4000858 History: Received November 24, 2009; Revised December 08, 2009; Published May 05, 2010; Online May 05, 2010

Bacterial cellulose (BC) and gelatin are well-known biomaterials. The novel bacterial cellulose/gelatin composite scaffolds were prepared using aqueous gelatin solution and bacterial cellulose excreted by Acetobacter xylinum. The prepared bacterial cellulose/gelatin scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, mechanical test, swelling, and thermal studies. The morphology of these bacterial cellulose/gelatin scaffolds indicated that the gelatin molecules could penetrate well between the individual nanofibers of the bacterial cellulose. With the incorporation of gelatin in the bacterial cellulose, the crystallinity index tended to decrease while the thermal stability was improved. After the incorporation of gelatin in the bacterial cellulose, Young’s modulus of the composite was increased from 3.7 GPa to 3.9 GPa, while the tensile strength and strain at break point were decreased from 170 MPa (7.5%) to 114 MPa (4%), respectively. The swelling behavior test indicated that the water uptake capacity of the composite was only half of the pure bacterial cellulose. Cell adhesion studies were carried out using 3T3 fibroblast cells. The cells incubated with BC/gelatin scaffolds for 48 h were capable of forming cell adhesion and proliferation. It showed much better biocompatibility than pure bacterial cellulose. So, the prepared BC/gelatin scaffolds are bioactive and may be suitable for cell adhesion/attachment, suggesting that these scaffolds can be used for wound dressing or tissue engineering scaffolds. Therefore, these novel BC/gelatin scaffolds are useful for biomedical applications.

FIGURES IN THIS ARTICLE
<>
Copyright © 2010 by American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.

References

Figures

Grahic Jump Location
Figure 1

Photograph of the BC pellicle in humid state (gel)

Grahic Jump Location
Figure 2

Schematic of BC/gelatin interaction

Grahic Jump Location
Figure 3

SEM images of the BC ((a) surface; (c) cross section) and BC/gelatin composite ((b) surface; (d) cross section)

Grahic Jump Location
Figure 4

FTIR spectra of BC, gelatin, and BC/gelatin composites

Grahic Jump Location
Figure 5

XRD patterns of the BC, gelatin, and BC/gelatin composites

Grahic Jump Location
Figure 6

TGA spectra of the BC, gelatin, and BC/gelatin composites

Grahic Jump Location
Figure 7

Swelling behavior of the BC and BC/gelatin composite in function of time for water

Grahic Jump Location
Figure 8

Stress-strain curves of the BC and BC/gelatin composite

Grahic Jump Location
Figure 9

SEM images of cross section morphology of fracture region ((a) BC; (b) BC/gelatin composite)

Grahic Jump Location
Figure 10

3T3 fibroblast cell attachments of (a) pure BC and (b) BC/gelatin scaffolds of 48 h seeding the cells

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In