To date, several methods have been used in microfluidic fabrication, including soft lithograph [3-5], photo-patterning [6-8], laser-based technologies [9,10], molding [11-13], and bioprinting [2,14-16]. However, due to their intrinsic characteristics, each of the above-mentioned technologies has its advantages and disadvantages. Soft lithography is the most popular method in microfluidic channel fabrication due to its low cost, accuracy, and reproducibility. Using soft lithography technology, Ling et al.  fabricated microfluidic cell-laden agarose hydrogel, which resulted in a significant increase in cell viability during media perfusion compared to static controls. Cuchiara et al.  developed a soft lithography process to fabricate a poly(ethylene glycol) diacrylate hydrogel microfluidic network. With media perfusion, encapsulated mammalian cells maintained a high viability rate in bulk hydrogel. However, soft lithograph is not a viable option for fabrication of complex three-dimensional (3D) constructs due to its cumbersome procedures. Despite their superior accuracy and repeatability, photo-patterning and laser-based methods may not be suitable for fabricating thick tissue constructs because of their limited light-penetrating depths in precursor solution. Offra et al.  proposed a focal laser photoablation capable of generating microstructures in transparent hydrogels. Cell behavior was successfully guided by the microchannel pattern. Molding is an inexpensive and scalable method, but complex 3D geometry is difficult to achieve and postprocedures are required after fabrication. In Ref. , Nazhat et al. used a molding method to incorporate unidirectionally aligned soluble phosphate-based glass fibers into dense collagen scaffolds. The diameters of the achieved microfluidic channels were around 30–40 μm, and a significant increase in cell viability was observed in the hydrogel sheets. Despite the plethora of work in microfluidic channel fabrication using the traditional methods, only a few researchers have developed strategies for bioprinting of microfluidic channels, where bioprinting can be defined as computer-controlled layer-by-layer bioadditive process enabling printing living cells precisely per predefined patterns . Cell encapsulated biomaterials can be directly patterned onto substrate without any pretreating steps (such as mold or mask preparation). It offers several advantages, including precise control [16,17], automated fabrication capability [18,19], and feasibility of achieving complex shapes . Zhao et al.  recently presented a methodology in bioprinting of perfused straight microfluidic channel structures in thick hydrogel. They created a temporary structure to form the hollow cavity which was then removed by a postprocess.