Biopolymeric micro and nanoparticles can be vital for various applications in industry, science and medicine. Such as one of the linear biopolymers, chitosan, has become the center of attention for medical and pharmaceutical applications in recent years due to its high biocompatibility and biodegradability [1,2]. Chitosan is a hydrophilic macromolecule which can form biodegradable nanoparticles for site specific delivery of vaccines, genes, drugs and other biomolecules in human body . Moreover, it is used as a topical dressing in wound management due to its hemostatic, stimulation of healing, antimicrobial, nontoxic, biocompatible, and biodegradable aspects . Chitosan has also been considered as a promising candidate for bone tissue engineering since it (i) causes minimal foreign body reactions, (ii) has the ability to be molded into various geometries and forms such as porous structures, and (iii) is suitable for cell ingrowth and osteoconduction . The conventional methods for the formation of chitosan nanoparticles include emulsion droplet coalescence, emulsion solvent diffusion, reverse micellar method, ionic gelation, polyelectrolyte complexation and desolvation . These techniques are batchwise systems, mostly have the principle of dropwise addition of the cross-linker molecules to the chitosan solution or chitosan solution to the cross-linker solution. These conventional techniques usually suffer from aggregation of nanoparticles and labor-intense requires a qualified personnel throughout the process due to the lack of automation. Moreover, reproducibility of the process may also be problematic. One alternative to overcome these issues is to synthesize the chitosan nanoparticles within the microchannels in a continuous flow. In this case, the flow hence the process can be performed in a more controlled manner, the aggregation can be reduced due to the continuous flow nature of the process, and the process can be run autonomously once the required solutions are loaded into the microfluidic channels. Many research groups have worked on microfluidics devices that can produce chitosan micro and nanostructures such as microfibers, microparticles, and nanoparticles [1,7-9]. The integration of microfluidic and particle technologies has given promising results for both inorganic and organic micro and nanoparticles considering the rapid and tunable mixing, adjustable flow rates, and mixing time result in narrow size distribution or distinct shapes with better control of nanoprecipitation process .