The study of cell mechanics requires tools which can apply or measure small forces at a small scale, matching the cell size. A number of techniques exist to mechanically probe living cells, including atomic force microscopy, magnetic twisting cytometry, optical trapping, micropipette aspiration, shear flow, and stretchable substrates . Scientists and biomedical Engineers began developing micropipette aspiration for cell mechanics studies since the 1970s. Due to its simplicity, micropipette aspiration is still a widely adopted technique to study cell mechanics. An extensive review article by Hochmuth published in 2000 on micropipette aspiration on living cells provided a general overview of using continuum liquid and solid models to measure material properties such as cortical tension, Young's modulus, and viscoelasticity of living cells . In this current review, we will first briefly revisit the working principle and the infrastructure of micropipette aspiration systems. Then, we will focus mainly on primary research works highlighting some applications of micropipette aspiration in studying cellular mechanics at both nano- and microscales and the recent technology advancement of the integration of micropipette aspiration with microfluidics.