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Research Papers

The Application of Micropipette Aspiration in Molecular Mechanics of Single Cells

[+] Author and Article Information
Lap Man Lee

Department of Mechanical Engineering,
University of Michigan,
2350 Hayward Street,
Ann Arbor, MI 48109-2125
e-mail: melmlee@umich.edu

Allen P. Liu

Department of Mechanical Engineering,
Biomedical Engineering, Cell and
Molecular Biology Program,
Biophysics Program,
University of Michigan,
2350 Hayward Street,
Ann Arbor, MI 48109-2125
e-mail: allenliu@umich.edu

1Corresponding author.

Manuscript received January 23, 2015; final manuscript received February 24, 2015; published online June 16, 2015. Assoc. Editor: Jianping Fu.

J. Nanotechnol. Eng. Med 5(4), 040902 (Nov 01, 2014) (6 pages) Paper No: NANO-15-1010; doi: 10.1115/1.4029936 History: Received January 23, 2015; Revised February 24, 2015; Online June 16, 2015

Micropipette aspiration is arguably the most classical technique in mechanical measurements and manipulations of single cells. Despite its simplicity, micropipette aspiration has been applied to a variety of experimental systems that span different length scales to study cell mechanics, nanoscale molecular mechanisms in single cells, bleb growth, and nucleus dynamics, to name a few. Enabled by micro/nanotechnology, several novel microfluidic devices have been developed recently with better accuracy, sensitivity, and throughput. Further technical advancements of microfluidics-based micropipette aspiration would have broad applications in both fundamental cell mechanics studies and for disease diagnostics.

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Figures

Grahic Jump Location
Fig. 2

Study of cell–cell interaction using micropipette aspiration for cell manipulations. (a) Measure of cadherin binding force between CHO cells and RBCs (Reproduced with permission from Elsevier–Cell Press [30]), (b) measurement of the bond strength of a single receptor–ligand bond pair (Reproduced with permission from PNAS [31]), and (c) trace of the adhesion force of P-selectin glycoprotein ligand 1 in leukocyte (Reproduced with permission from Journal of Cell Science–The Company of Biologists [32]).

Grahic Jump Location
Fig. 1

Experimental setup of a traditional micropipette aspiration system

Grahic Jump Location
Fig. 3

Molecular mechanics of single cell by micropipette aspiration. (a) Bleb-based cell motility (Reproduced with permission from PNAS [34]), (b) organization of cytoskeletal proteins (Reproduced with permission from PNAS [33]), and (c) measurement of cortical tension on cell blebbing induced by laser ablations (Reproduced with permission from Nature Materials–Nature Publishing Group [38]).

Grahic Jump Location
Fig. 4

Micropipette aspiration enabled by microfluidics (a) a serial micropipette array to study breast cancer viscoelastic deformation (Reproduced with permission from Integrative Biology–RSC Society [43]), (b) a microfluidic plunger device to study the deformability of RBCs (Reproduced with permission from Lab on a Chip–RSC Society [45]), and (c) a μFPA to measure mechanical properties of cancer cells (Reproduced with permission from Lab on a Chip–RSC Society [46])

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