0
Research Papers

Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells

[+] Author and Article Information
Jingru Yi

Department of Electronic Science and Technology,
University of Science and Technology of China,
Road JinZhai 96,
Hefei 230027, China

Gang Zhao

Department of Electronic Science and Technology,
University of Science and Technology of China,
Road JinZhai 96,
Hefei 230027, China
Anhui Provincial Engineering Technology
Research Center for Biopreservation and
Artificial Organs,
Hefei 230027, China
e-mail: zhaog@ustc.edu.cn

1Corresponding author.

Manuscript received July 25, 2014; final manuscript received November 26, 2014; published online June 16, 2015. Assoc. Editor: Jianping Fu.

J. Nanotechnol. Eng. Med 5(4), 040904 (Nov 01, 2014) (7 pages) Paper No: NANO-14-1050; doi: 10.1115/1.4029331 History: Received July 25, 2014; Revised November 26, 2014; Online June 16, 2015

The effect of nanoparticles on subzero biotransport phenomena of living cells is very rare in the literature, although the information is of great importance for the application of nanotechnology in the field of cryobiology. In this study, subzero water transport phenomena in freezing HeLa cells in 1 × phosphate buffered saline (PBS) containing 0%, 0.05%, and 0.1% (w/w) hydroxyapatite (HA) nanoparticles with and without pre-incubation at 37 °C was quantitatively investigated. The results reveal that the presence of HA nanoparticles slightly facilitates the subzero water transport of HeLa cells.

FIGURES IN THIS ARTICLE
<>
Copyright © 2014 by ASME
Your Session has timed out. Please sign back in to continue.

References

Mazur, P., 1984, “Freezing of Living Cells—Mechanisms and Implications,” Am. J. Physiol., 247(3), pp. C125–C142. [PubMed]
Karlsson, J. O. M., and Toner, M., 1996, “Long-Term Storage of Tissues by Cryopreservation: Critical Issues,” Biomaterials, 17(3), pp. 243–256. [CrossRef] [PubMed]
He, X., 2011, “Thermostability of Biological Systems: Fundamentals, Challenges, and Quantification,” Open Biomed. Eng. J., 5, pp. 47–73. [CrossRef] [PubMed]
Yan, J. F., and Liu, J., 2008, “Nanocryosurgery and Its Mechanisms for Enhancing Freezing Efficiency of Tumor Tissues,” Nanomed.-Nanotechnol. Biol. Med., 4(1), pp. 79–87. [CrossRef]
Jing, L., and Zhong-Shan, D., 2009, “Nano-Cryosurgery: Advances and Challenges,” J. Nanosci. Nanotechnol., 9(8), pp. 4521–4542. [CrossRef] [PubMed]
Yang, G., Veres, M., Szalai, G., Zhang, A. L., Xu, L. X., and He, X. M., 2011, “Biotransport Phenomena in Freezing Mammalian Oocytes,” Ann. Biomed. Eng., 39(1), pp. 580–591. [CrossRef] [PubMed]
Toner, M., 1993, “Nucleation of Ice Crystals Inside Biological Cells,” Advances in Low-Temperature Biology, JAI Press Ltd., London, UK.
Zhang, W. J., Gilstrap, K., Wu, L. Y., Bahadur, K. C. R., Moss, M. A., Wang, Q. A., Lu, X. B., and He, X. M., 2010, “Synthesis and Characterization of Thermally Responsive Pluronic F127-Chitosan Nanocapsules for Controlled Release and Intracellular Delivery of Small Molecules,” ACS Nano, 4(11), pp. 6747–6759. [CrossRef] [PubMed]
Rao, W., Zhang, W. J., Poventud-Fuentes, I., Wang, Y. C., Lei, Y. F., Agarwal, P., Weekes, B., Li, C. L., Lu, X. B., Yu, J. H., and He, X. M., 2014, “Thermally Responsive Nanoparticle-Encapsulated Curcumin and Its Combination With Mild Hyperthermia for Enhanced Cancer Cell Destruction,” Acta Biomater., 10(2), pp. 831–842. [CrossRef] [PubMed]
Schmidt, G., and Malwitz, M. M., 2003, “Properties of Polymer–Nanoparticle Composites,” Curr. Opin. Colloid Interface Sci., 8(1), pp. 103–108. [CrossRef]
Gilstrap, K., Hu, X. X., Lu, X. B., and He, X. M., 2011, “Nanotechnology for Energy-Based Cancer Therapies,” Am. J. Cancer Res., 1(4), pp. 508–520. [PubMed]
Di, D. R., He, Z. Z., Sun, Z. Q., and Liu, J., 2012, “A New Nano-Cryosurgical Modality for Tumor Treatment Using Biodegradable MgO Nanoparticles,” Nanomed.-Nanotechnol. Biol. Med., 8(8), pp. 1233–1241. [CrossRef]
Hwang, Y. J., Ahn, Y. C., Shin, H. S., Lee, C. G., Kim, G. T., Park, H. S., and Lee, J. K., 2006, “Investigation on Characteristics of Thermal Conductivity Enhancement of Nanofluids,” Curr. Appl. Phys., 6(6), pp. 1068–1071. [CrossRef]
Lv, F. K., Liu, B. L., Li, W. J., and Jaganathan, G. K., 2014, “Devitrification and Recrystallization of Nanoparticle-Containing Glycerol and PEG-600 Solutions,” Cryobiology, 68(1), pp. 84–90. [CrossRef] [PubMed]
Hao, B., and Liu, B., 2011, “Thermal Properties of PVP Cyroprotectants With Nanoparticles,” ASME J. Naontech. Eng. Med.2(2), pp. 021015. [CrossRef]
Guha, A., and Devireddy, R. V., 2010, “Effect of Palmitoyl Nanogold Particles on the Subzero Thermal Properties of Phosphate Buffered Saline Solutions,” ASME J. Nanotechnol. Eng. Med., 1(2), p. 021004. [CrossRef]
Bissoyi, A., Pramanik, K., Panda, N. N., and Sarangi, S. K., 2014, “Cryopreservation of hMSCs Seeded Silk Nanofibers Based Tissue Engineered Constructs,” Cryobiology, 68(3), pp. 332–342. [CrossRef] [PubMed]
Zhu, S. H., Huang, B. Y., Zhou, K. C., Huang, S. P., Liu, F., Li, Y. M., Xue, G., and Long, Z. G., 2004, “Hydroxyapatite Nanoparticles as a Novel Gene Carrier,” J. Nanopart. Res., 6(2), pp. 307–311. [CrossRef]
Jiang, L. X., Jiang, L. Y., Xu, L. J., Han, C. T., and Xiong, C. D., 2014, “Effect of a New Surface-Grafting Method for Nano-Hydroxyapatite on the Dispersion and the Mechanical Enhancement for Poly(Lactide-co-Glycolide),” eXPRESS Polym. Lett., 8(2), pp. 133–141. [CrossRef]
Runrong, L., Xuan, M., Qicong, Y., and Baihua, T., 2007, “Preparation of Bioactive Nano-Hydroxyapatite Coating for Artificial Cornea,” Curr. Appl. Phys., 7, pp. e85–e89. [CrossRef]
Devireddy, R. V., Raha, D., and Bischof, J. C., 1998, “Measurement of Water Transport During Freezing in Cell Suspensions Using a Differential Scanning Calorimeter,” Cryobiology, 36(2), pp. 124–155. [CrossRef] [PubMed]
Kleinhans, F. W., and Mazur, P., 2007, “Comparison of Actual Vs. Synthesized Ternary Phase Diagrams for Solutes of Cryobiological Interest,” Cryobiology, 54(2), pp. 212–222. [CrossRef] [PubMed]
Mazur, P., 1963, “Kinetics of Water Loss From Cells at Subzero Temperatures and Likelihood of Intracellular Freezing,” J. Gen. Physiol., 47(2), pp. 347–369. [CrossRef] [PubMed]
Karlsson, J. O. M., 2010, “Effects of Solution Composition on the Theoretical Prediction of Ice Nucleation Kinetics and Thermodynamics,” Cryobiology, 60(1), pp. 43–51. [CrossRef] [PubMed]
Berrada, M. S., and Bischof, J. C., 2001, “Evaluation of Freezing Effects on Human Microvascular-Endothelial Cells (HMEC),” Cryoletters, 22(6), pp. 353–366. [PubMed]
Yang, C. Y., Yeh, Y. H. F., Lee, P. T., and Lin, T. T., 2013, “Effect of Cooling Rate and Cryoprotectant Concentration on Intracellular Ice Formation of Small Abalone (Haliotis Diversicolor) Eggs,” Cryobiology, 67(1), pp. 7–16. [CrossRef] [PubMed]
Sudhakaran, P. R., Prinz, R., and Vonfigura, K., 1982, “Effect of Temperature on Endocytosis and Degradation of Sulfated Proteoglycans by Cultured Skin Fibroblasts,” J. Biosci., 4(4), pp. 413–418. [CrossRef]

Figures

Grahic Jump Location
Fig. 2

Sedimentation of nanoparticle suspensions at different time points. Arrows indicate the aggregation of the HA nanoparticles. 0 h, 0.5 h, 1 h, 1.5 h, and 2 h.

Grahic Jump Location
Fig. 1

The radius of particles with regard to the weight ratio of Na6(PO3)6 to HA. Each value was automatically averaged (n  > 10) and calculated by the machine software according to the preset property of the solution (1 × PBS) and particles (assumed to be circular).

Grahic Jump Location
Fig. 4

Results obtained from individual curve fitting. (a) Representative normalized cell volume data (circles) with their individual fitting curves (solid lines) and (b) transmembrane water permeability parameters obtained from individual curve fitting and related statistical analysis results. Error bar: SD.

Grahic Jump Location
Fig. 3

Volumetric responses of HeLa cells at various temperatures during freezing at 15 °C min−1. (a) Cells in 1 × PBS, (b) cells in 1 × PBS with 0.05% HA nanoparticle suspensions without incubation, and (c) cells in 0.1% HA nanoparticles solutions without incubation.

Grahic Jump Location
Fig. 5

Results obtained from combined curve fitting. (a) Normalized cell volume at different HA concentrations with and without incubation (symbols). Solid lines show the combined fitting curves. Error bar indicates the standard error of mean. (b) Transmembrane water permeability parameters. (c) Arrhenius plot of water permeability of HeLa cells membrane (Lp) at different HA concentrations with and without incubation.

Grahic Jump Location
Fig. 7

SEM images of HeLa cells in 1 × PBS with 0–0.1% HA nanoparticles either with or without incubation at 37 °C. Arrows indicate the HA nanoparticles clusters on the cell membrane. (a) 1 × PBS, (b) 1 × PBS/0.05% HA, (c) 1 × PBS/0.1% HA, (d) 1 × PBS/0.05% HA/incubation, and (e) 1 × PBS/0.1% HA/incubation.

Grahic Jump Location
Fig. 6

Predicted subzero water transport of HeLa cells cooled at 10, 30, 60, and 100 °C min−1. Here Vb is set as 0.35 V0.

Tables

Errata

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In