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Research Papers: Fractal Engineering and Biomedicine

The Improvement of Antimicrobial Activity of Kanamycin and Ciprofloxacin Antibiotics Coupled With Biocompatible Magnetite Nanoparticles and Characterization of Their Structure

[+] Author and Article Information
Ulviyya Alimammad Hasanova

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: ulviyya3@rambler.ru

Mahammadali Ahmad Ramazanov

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: mamed_r50@mail.ru

Abel Mammadali Maharramov

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: rector@bsu.az

Sarvinaz Faiq Hajiyeva

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: sarvinazhajiyeva@gmail.com

Yana Vacheslav Parfyonova

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: joanna845@yandex.ru

Goncha Malik Eyvazova

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: eygoncha@gmail.com

Flora Vidadi Hajiyeva

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: flora_1985@mail.ru

Narmina Arthur Guliyeva

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: mamedr@physic.ab.az

Solmaz Bayram Veliyeva

Nano Research Laboratory,
Baku State University,
Z. Khalilov 23,
Baku AZ1148, Azerbaijan
e-mail: nanomaterials@bsu.edu.az

Manuscript received November 23, 2015; final manuscript received March 16, 2016; published online May 18, 2016. Assoc. Editor: Charalabos Doumanidis.

J. Nanotechnol. Eng. Med 6(4), 041006 (May 18, 2016) (7 pages) Paper No: NANO-15-1094; doi: 10.1115/1.4033126 History: Received November 23, 2015; Revised March 16, 2016

In this paper, we present the synthesis of nanostructures of magnetite nanoparticles (NPs) with ciprofloxacin and kanamycin antibiotics, based on self-assembling principle. The nanostructures were prepared in crystallite size, ranging 8–16 nm, in one pot addition setup and further washing steps, using only iron precursors and above-mentioned antibiotics as stabilizers. Nanostructures were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis methods, Fourier transform infrared (FTIR) and ultraviolet (UV) spectroscopy methods. It was found that they have well-shaped spherical form and are homogeneous in size. The quantitative analysis of nanostructured antibiotics was performed by atom absorbance spectroscopy (AAS) as well as on the basis of Lambert–Beer law. Prepared nanostructures were tested on Staphylococcus aureus and Pseudomonas aeruginosa. Obtained results demonstrated that these nanostructures are able to improve antimicrobial properties and decrease the minimal inhibitory concentration (MIC) of pristine kanamycin and ciprofloxacin antibiotics.

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Figures

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Fig. 1

The chemical structure of (a) kanamycin 2-(aminomethyl)-6-[4,6-diamino-3-[4-amino-3,5-dihydroxy-6-(hydroxymethyl) tetrahydropyran-2-yl]oxy-2-hydroxy-cyclohexoxy]-tetrahydropyran-3,4,5-triol and (b) ciprofloxacin 1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-quinoline-3-carboxylic acid

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Fig. 2

XRD pattern of Fe3O4@KNM NPs

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Fig. 3

XRD pattern of Fe3O4@CFX NPs

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Fig. 4

FTIR spectra (a) pristine kanamycin, (b) Fe3O4@KNM, and (c) pristine Fe3O4

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Fig. 5

FTIR spectra (a) pristine ciprofloxacin, (b) Fe3O4@CFX, and (c) pristine Fe3O4

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Fig. 6

UV spectra of pure ciprofloxacin (1) 4.5 μg ml−1; (2) 5 μg ml−1; (3) 6 μg ml−1; (4) 8 μg ml−1 (5) 8.5 μg ml−1; (6) 10 μg ml−1; and (7) Fe3O4@CFX found 9 μg ml−1

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Fig. 7

(a) SEM image of Fe3O4@KNM NPs and (b) ED pattern of Fe3O4@KNM NPs

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Fig. 8

(a) SEM image of Fe3O4@CFX NPs and (b) ED pattern of Fe3O4@CFX NPs

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Fig. 9

MIC of kanamycin and Fe3O4@KNM NPs against S. aureus are, respectively, 5 μg ml−1 and 1 μg ml−1

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Fig. 10

The graphic representation of inhibition of P. aeruginosa biofilm development in the presence of kanamycin and Fe3O4@KNM NPs

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Fig. 11

The graphic representation of inhibition of S. aureus biofilm development in the presence of kanamycin and Fe3O4@KNM NPs

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Fig. 12

MIC of ciprofloxacin and Fe3O4@CFX NPs against S. aureus and P. aeruginosa equal to 0,1 μg ml−1

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Fig. 13

The graphic representation of inhibition of P. aeruginosa biofilm development in the presence of ciprofloxacin and Fe3O4@CFX NPs

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Fig. 14

The graphic representation of inhibition of S. aureus biofilm development in the presence of ciprofloxacin and Fe3O4@CFX NPs

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