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Research Papers: Regular Papers

On-Chip Cytotoxicity Testing of Nepali Chiya Extract

[+] Author and Article Information
Sueon Kim

Department of Mechanical Engineering,
College of Engineering,
Kyung Hee University,
1732, Deogyeongdaero, Giheung-gu,
Yongin-si 17104, South Korea
e-mail: sueon1989@khu.ac.kr

In Hyuk Jang

Department of Mechanical Engineering,
College of Engineering,
Kyung Hee University,
1732, Deogyeongdaero, Giheung-gu,
Yongin-si 17104, South Korea
e-mail: inhyuk87@khu.ac.kr

Won Gu Lee

Department of Mechanical Engineering,
College of Engineering,
Kyung Hee University,
1732, Deogyeongdaero, Giheung-gu,
Yongin-si 17104, South Korea
e-mail: termylee@khu.ac.kr

1Corresponding author.

Manuscript received September 15, 2015; final manuscript received March 22, 2016; published online May 3, 2016. Assoc. Editor: Feng Xu.

J. Nanotechnol. Eng. Med 6(4), 041004 (May 03, 2016) (5 pages) Paper No: NANO-15-1078; doi: 10.1115/1.4033194 History: Received September 15, 2015; Revised March 22, 2016

Here, we report a threshold limit value (TLV) of on-chip cytotoxicity of Nepali Chiya, the Nepali traditional black tea. To demonstrate our proof-of-concept validation, we used the active sealing chip with serial dilution that can directly perform on-chip cytotoxicity testing onto the cells cultured in a petri dish. In our experiments, the TLV for mortality on HeLa cells was observed as 400 μg/ml for Nepali Chiya extract. We believe this approach would be a rapid and simple method for on-chip TLV screening of potability of tea extract at the laboratory level, and furthermore as a new potential drug supplement in pharmaceutical industries.

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Figures

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Fig. 1

(a) Experimental results for serial dilution test, (b) Photograph of serial dilution experiments for quantitative analysis, and (c) Graph of intensity according to each well port

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Fig. 2

(a) Experimental results of the direct bonding of the microfluidic cell chip onto the surface of the cultured cells, (b) A boundary region for active sealing, and (c) Morphology of hydrogen peroxide solution treated HeLa cells. The cells were photographed 3 hrs later.

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Fig. 3

(a) Graph of cell viability on Nepali Chiya extracts concentration on HeLa cells and (b) Graph of cell viability by hydrogen peroxide concentration on HeLa cells

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Fig. 4

Graph of cell viability by Nepali Chiya concentration with/without hydrogen peroxide on HeLa cells

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