Research Papers

Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells

[+] Author and Article Information
Jingru Yi

Department of Electronic Science and Technology,
University of Science and Technology of China,
Road JinZhai 96,
Hefei 230027, China

Gang Zhao

Department of Electronic Science and Technology,
University of Science and Technology of China,
Road JinZhai 96,
Hefei 230027, China
Anhui Provincial Engineering Technology
Research Center for Biopreservation and
Artificial Organs,
Hefei 230027, China
e-mail: zhaog@ustc.edu.cn

1Corresponding author.

Manuscript received July 25, 2014; final manuscript received November 26, 2014; published online June 16, 2015. Assoc. Editor: Jianping Fu.

J. Nanotechnol. Eng. Med 5(4), 040904 (Nov 01, 2014) (7 pages) Paper No: NANO-14-1050; doi: 10.1115/1.4029331 History: Received July 25, 2014; Revised November 26, 2014; Online June 16, 2015

The effect of nanoparticles on subzero biotransport phenomena of living cells is very rare in the literature, although the information is of great importance for the application of nanotechnology in the field of cryobiology. In this study, subzero water transport phenomena in freezing HeLa cells in 1 × phosphate buffered saline (PBS) containing 0%, 0.05%, and 0.1% (w/w) hydroxyapatite (HA) nanoparticles with and without pre-incubation at 37 °C was quantitatively investigated. The results reveal that the presence of HA nanoparticles slightly facilitates the subzero water transport of HeLa cells.

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Grahic Jump Location
Fig. 1

The radius of particles with regard to the weight ratio of Na6(PO3)6 to HA. Each value was automatically averaged (n  > 10) and calculated by the machine software according to the preset property of the solution (1 × PBS) and particles (assumed to be circular).

Grahic Jump Location
Fig. 2

Sedimentation of nanoparticle suspensions at different time points. Arrows indicate the aggregation of the HA nanoparticles. 0 h, 0.5 h, 1 h, 1.5 h, and 2 h.

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Fig. 3

Volumetric responses of HeLa cells at various temperatures during freezing at 15 °C min−1. (a) Cells in 1 × PBS, (b) cells in 1 × PBS with 0.05% HA nanoparticle suspensions without incubation, and (c) cells in 0.1% HA nanoparticles solutions without incubation.

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Fig. 4

Results obtained from individual curve fitting. (a) Representative normalized cell volume data (circles) with their individual fitting curves (solid lines) and (b) transmembrane water permeability parameters obtained from individual curve fitting and related statistical analysis results. Error bar: SD.

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Fig. 5

Results obtained from combined curve fitting. (a) Normalized cell volume at different HA concentrations with and without incubation (symbols). Solid lines show the combined fitting curves. Error bar indicates the standard error of mean. (b) Transmembrane water permeability parameters. (c) Arrhenius plot of water permeability of HeLa cells membrane (Lp) at different HA concentrations with and without incubation.

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Fig. 6

Predicted subzero water transport of HeLa cells cooled at 10, 30, 60, and 100 °C min−1. Here Vb is set as 0.35 V0.

Grahic Jump Location
Fig. 7

SEM images of HeLa cells in 1 × PBS with 0–0.1% HA nanoparticles either with or without incubation at 37 °C. Arrows indicate the HA nanoparticles clusters on the cell membrane. (a) 1 × PBS, (b) 1 × PBS/0.05% HA, (c) 1 × PBS/0.1% HA, (d) 1 × PBS/0.05% HA/incubation, and (e) 1 × PBS/0.1% HA/incubation.



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