Application of low-magnitude strains to cells on small-thickness scaffolds, such as those for rodent calvarial defect models, is problematic, because general translation systems have limitations in terms of generating low-magnitude smooth signals. To overcome this limitation, we developed a cyclic strain generator using a customized, flexure-based, translational nanoactuator that enabled generation of low-magnitude smooth strains at the subnano- to micrometer scale to cells on small-thickness scaffolds. The cyclic strain generator we developed showed predictable operational characteristics by generating a sinusoidal signal of a few micrometers (4.5 μm) without any distortion. Three-dimensional scaffolds fitting the critical-size rat calvarial defect model were fabricated using poly(caprolactone), poly(lactic-co-glycolic acid), and tricalcium phosphate. Stimulation of human adipose–derived stem cells (ASCs) on these fabricated scaffolds using the cyclic strain generator we developed resulted in upregulated osteogenic marker expression compared to the nonstimulated group. These preliminary in vitro results suggest that the cyclic strain generator successfully provided mechanical stimulation to cells on small-thickness scaffolds, which influenced the osteogenic differentiation of ASCs.
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POSTECH,
Korea Polytechnic University,
Siheung 429-793,
POSTECH, Pohang 790-751,
POSTECH,
College of Medicine,
The Catholic University of Korea,
POSTECH,
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November 2013
Technical Briefs
Flexure-Based Device for Cyclic Strain-Mediated Osteogenic Differentiation
Kyung Shin Kang,
POSTECH,
Kyung Shin Kang
1
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
1These authors contributed equally to this work.
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Young Hun Jeong,
Korea Polytechnic University,
Siheung 429-793,
Young Hun Jeong
1
Department of Mechanical Engineering
,Korea Polytechnic University,
Siheung 429-793,
South Korea
1These authors contributed equally to this work.
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Jung Min Hong,
POSTECH, Pohang 790-751,
Jung Min Hong
Department of Mechanical Engineering
,POSTECH, Pohang 790-751,
South Korea
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Woon-Jae Yong,
POSTECH,
Woon-Jae Yong
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
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Jong-Won Rhie,
College of Medicine,
The Catholic University of Korea,
Jong-Won Rhie
Department of Plastic Surgery
,College of Medicine,
The Catholic University of Korea,
Seoul 137-701, South Korea
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Dong-Woo Cho
POSTECH,
Dong-Woo Cho
2
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
2Correspondence to: Dong-Woo Cho, Ph.D., Department of Mechanical Engineering, POSTECH, Center for Rapid Prototyping Based 3D Tissue/Organ Printing, POSTECH, San 31, Hyoja-dong, Nam-gu, Pohang, 790-751, Republic of Korea, e-mail: dwcho@postech.ac.kr
Search for other works by this author on:
Kyung Shin Kang
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
Young Hun Jeong
Department of Mechanical Engineering
,Korea Polytechnic University,
Siheung 429-793,
South Korea
Jung Min Hong
Department of Mechanical Engineering
,POSTECH, Pohang 790-751,
South Korea
Woon-Jae Yong
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
Jong-Won Rhie
Department of Plastic Surgery
,College of Medicine,
The Catholic University of Korea,
Seoul 137-701, South Korea
Dong-Woo Cho
Department of Mechanical Engineering
,POSTECH,
Pohang 790-751, South Korea
1These authors contributed equally to this work.
2Correspondence to: Dong-Woo Cho, Ph.D., Department of Mechanical Engineering, POSTECH, Center for Rapid Prototyping Based 3D Tissue/Organ Printing, POSTECH, San 31, Hyoja-dong, Nam-gu, Pohang, 790-751, Republic of Korea, e-mail: dwcho@postech.ac.kr
Contributed by the Bioengineering Division of ASME for publication in the Journal of Biomechanical Engineering. Manuscript received November 15, 2012; final manuscript received July 5, 2013; accepted manuscript posted July 29, 2013; published online September 23, 2013. Assoc. Editor: James C. Iatridis.
J Biomech Eng. Nov 2013, 135(11): 114501 (8 pages)
Published Online: September 23, 2013
Article history
Received:
November 15, 2012
Revision Received:
July 5, 2013
Accepted:
July 29, 2013
Citation
Shin Kang, K., Hun Jeong, Y., Min Hong, J., Yong, W., Rhie, J., and Cho, D. (September 23, 2013). "Flexure-Based Device for Cyclic Strain-Mediated Osteogenic Differentiation." ASME. J Biomech Eng. November 2013; 135(11): 114501. https://doi.org/10.1115/1.4025103
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