Abstract
Six red blood cell (RBC) antigen systems, coupled with human lymphocyte antigen (HLA) phenotyping, were used to establish paternity on 28 mother/child/allegedfather trios. Samples were subsequently examined using the deoxyribonucleic acid (DNA) fingerprinting test with the multilocus Jeffreys DNA probes 33.6 and 33.15. In 27 of 28 paternity cases, the DNA fingerprinting test results supported and enhanced the results of RBC and HLA typing by resolving disputed paternity cases conclusively. One discrepancy between conventional serological methods and DNA analysis is discussed.
Issue Section:
Research Papers
References
1.
Krause
, H. J.
, Abbott
, J. F.
, Miale
, J. B.
, Sell
, K. W.
, Jennings
, E. R.
, and Rettberg
, W. A. H.
, “Joint AMA-ABA Guidelines: Present Status of Serologic Testing in Problems of Disputed Parentage
,” Family Law Quarterly
, Vol. 10
, No. 3
, 1976
, pp. 247
-285
.2.
Schacter
, B. Z.
, Hsu
, S. H.
, and Bias
, W. B.
, “HLA and Other Genetic Markers in Disputed Paternity: A Report of 50 Cases
,” Transplantation Proceedings
0041-1345, Vol. 9
, 1977
, pp. 233
-237
.3.
Houtz
, T. D.
, Brooks
, M. A.
, Wenk
, R. E.
, and Dawson
, R. B.
, “Utility of HLA and Six Erythrocyte Antigen Systems in Excluding Paternity Among 500 Disputed Cases
,” Forensic Science International
0379-0738, Vol. 17
, 1981
, pp. 211
-218
.4.
Singh
, G.
, Johns
, M. M.
, and Paul
, G.
, “Paternity Testing: Analysis of Six Blood Groups and HLA Markers, with Particular Reference to Comparison of Races
,” American Journal of Clinical Pathology
0002-9173, Vol. 78
, No. 5
, 1982
, pp. 748
-752
.5.
Dykes
, D. D.
, “The Use of Frequency Tables in Parentage Testing
,” Probability of Inclusion in Paternity Testing: A Technical Workshop
, H.
Silver
, Ed., American Association of Blood Banks
, Arlington, VA
, 1982
, pp. 15
-44
.6.
Heise
, E. R.
, Keever
, C.
, and McMahan
, M. R.
, “A Critical Analysis of Paternity Determination Using HLA and Five Erythrocyte Antigen Systems
,” American Journal of Forensic Medicine and Pathology
0195-7910, Vol. 4
, No. 1
, 03
1983
, pp. 15
-23
.7.
Davey
, F. R.
, Hubbell
, C. A.
, Lauenstein
, K. J.
, Tinnesz
, C.
, and Henry
, J. B.
, “Analysis of Paternity: The Use of HLA and Red Cell Antigens
,” Transfusion
0372-1248, Vol. 24
, No. 4
, 1984
, pp. 340
-342
.8.
Hilderson
, Y.
and Henry
, M. R.
, “Scientific Testing for Paternity Establishment
,” Paternity Establishment
, 2nd ed., U.S. Department of Health and Human Services
, Washington, DC
, 11
1985
, p. 15.9.
Jeffreys
, A. J.
, Wilson
, V.
, and Thein
, S. L.
, “Hypervariable Minisatellite Regions in Human DNA
,” Nature
0028-0836, Vol. 314
, No. 7
, 03
1985
, pp. 67
-73
.10.
Jeffreys
, A. J.
, Wilson
, V.
, and Thein
, S. L.
, “Individual-Specific ‘Fingerprints’ of Human DNA
,” Nature
0028-0836, Vol. 316
, No. 4
, 07
1985
, pp. 76
-79
.11.
Jeffreys
, A. J.
, Brookfield
, F. Y.
, and Semeonoff
, R.
, “Positive Identification of an Immigration Test-Case Using Human DNA Fingerprints
,” Nature
0028-0836, Vol. 317
, 10
1985
, pp. 818
-819
.12.
Gill
, P.
, Jeffreys
, A. J.
, and Werrett
, D. J.
, “Forensic Application of DNA ‘Fingerprints’
,” Nature
0028-0836, Vol. 318
, 12
1985
, pp. 577
-579
.13.
Widmann
, F. K.
, Ed., Technical Manual
, 9th ed., American Association of Blood Banks
, Arlington, VA
, 1985
, pp. 113
-169
.14.
“
NIH Lymphocytotoxicity Technique
,” NIAID Manual of Tissue Typing Techniques, 1979–1980
, J. G.
Ray
, Ed., NIH Publication No. 80-545, National Institutes of Health
, Bethesda, MD
, 1979
, pp. 39
-41
.15.
Walker
, R. H.
, “Probability in the Analysis of Paternity Test Results
,” Paternity Testing
, H.
Silver
, Ed., American Association of Blood Banks
, Washington, DC
, 1978
, pp. 69
-135
.16.
Hummel
, K.
, “On the Theory and Practice of Essen-Moeller's W Value and Gurtler's Paternity Index (PI)
,” Forensic Science International
0379-0738, Vol. 25
, 1984
, pp. 1
-17
.17.
Southern
, E. M.
, “Gel Electrophoresis of Restriction Fragments
,” Methods in Enzymology
, Vol. 68
, 1980
, pp. 152
-176
.18.
Feinberg
, A. P.
and Vogelstein
, B.
, “A Technique for Radiolabelling DNA Restriction Endonuclease Fragments to High Specific Activity
,” Analytical Biochemistry
0003-2697, Vol. 132
, 1983
, pp. 6
-13
.19.
Jeffreys
, A. J.
, Royle
, N. J.
, Wilson
, V.
, and Wong
, Z.
, “Spontaneous Mutation Rates to New Length Alleles at Tandem-Repetitive Hypervariable Loci in Human DNA
,” Nature
0028-0836, Vol. 332
, 03
1988
, pp. 278
-281
.20.
Jeffreys
, A. J.
, Wilson
, V.
, Thein
, S. L.
, Weatherall
, D. J.
, and Ponder
, B. A. J.
, “DNA ‘Fingerprints’ and Segregation Analysis of Multiple Markers in Human Pedigrees
,” American Journal of Human Genetics
0002-9297, Vol. 39
, 1986
, pp. 11
-24
.21.
Jeffreys
, A. J.
, “Highly Variable Minisatellites and DNA Fingerprints
,” Biochemical Society Transactions
0300-5127, Vol. 15
, 1987
, pp. 309
-317
.22.
Chimera
, J. A.
, Litt
, M.
, and Dykes
, D.
, “Use of a Highly Polymorphic DNA Sequence in Paternity Evaluation
,” Genome
0831-2796, Vol. 30
, 08
1988
, p. 233.23.
Wong
, Z.
, Wilson
, V.
, Patel
, I.
, Povey
, S.
, and Jeffreys
, A. J.
, “Characterization of a Panel of Highly Variable Minisatellites Cloned from Human DNA
,” Annals of Human Genetics
0003-4800, Vol. 51
, 1987
, pp. 269
-288
.24.
Langaney
, A.
and Pison
, G.
, “Probability of Paternity: Useless
,” American Journal of Human Genetics
0002-9297, Vol. 27
, 1975
, pp. 558
-561
.25.
Ellman
, I. M.
and Kaye
, D.
, “Probabilities and Proof: Can HLA and Blood Group Testing Prove Paternity?
” New York University Law Review
, Vol. 54
, 1979
, pp. 1131
-1162
.26.
Jaffe
, L. R.
, “Comment on the Judicial Use of HLA Paternity Test Results and Other Statistical Evidence: A Response to Terasaki
,” Journal of Family Law
, Vol. 17
, 1978–1979, pp. 457
-485
.27.
Aickin
, M.
, “Some Fallacies in the Computation of Paternity Probabilities
,” American Journal of Human Genetics
0002-9297, Vol. 36
, 1984
, pp. 904
-915
.28.
Li
, C. C.
and Chakravarti
, A.
, “Basic Fallacies in the Formulation of the Paternity Index
,” American Journal of Human Genetics
0002-9297, Vol. 37
, 1985
, pp. 809
-818
.29.
Kanter
, E.
, Baird
, M.
, Pasquale
, S.
, and Balazs
, I.
, “Analysis of Restriction Fragment Polymorphisms in DNA Recovered from Dried Blood
,” Journal of Forensic Sciences
0022-1198, Vol. 31
, No. 2
, 04
1986
, pp. 403
-408
.30.
Giusti
, A.
, Baird
, M.
, Pasquale
, S.
, Balazs
, I.
, and Glassberg
, J.
, “Application of DNA Polymorphisms to the Analysis of DNA Recovered from Semen
,” Journal of Forensic Sciences
0022-1198, Vol. 31
, No. 2
, 04
1986
, pp. 409
-417
.
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