Abstract
According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure inhouse. Seven phases were designed to validate the use of the AmpFℓSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.
References
1.
Budowle
B
, Technical Working Group on DNA Analysis Methods. Guidelines for a quality assurance program for DNA analysis
. Crime Lab Digest
1995
;22
:21
-43
.2.
Baylor College of Medicine, inventors
. PE Applied Biosystems Corporation, assignee
. AmpFlSTR Profiler Plus PCR Amplification Kit
. US Patent 5,364,759.3.
Hammond
H A
, Jin
L
, Zhong
Y
, Caskey
C T
, Chakraborty
R
. Evaluation of 13 short tandem repeat loci for use in personal identification applications
. Am J Hum Genet
1994
;55
:175
-89
.4.
Fregeau
C J
, Bowen
K L
, Fourney
R M
. Validation of highly polymorphic fluorescent multiplex short tandem repeat systems using two generations of DNA sequencers
. J Forensic Sci
1999
;44
:133
-66
.5.
Perkin-Elmer Applied Biosystems AmpFlSTR Profiler Plus PCR Amplification Kit User's Manual
, 1997
.6.
Wallin
J M
, Buoncristiani
M R
, Lazuruk
K D
, Fildes
N
, Holt
C L
, Walsh
P S
. TWGDAM validation of the AmpFlSTR Blue PCR amplification kit for forensic casework analysis
. J Forensic Sci
1998
;43
:854
-70
.7.
Baylor College of Medicine, investors
. PE Applied Biosystems Corporation, assignee
. AmpFlSTR Blue PCR Amplification Kit
. US Patent 5,364,759.8.
Baylor College of Medicine, inventors
. Promega Corporation, assignee. GenePrint PowerPlex Fluorescent STR System.9.
Genescan and Genotyper [computer programs] Macintosh version,
1997
.10.
STaR CALL [computer program] for Hitachi FMBIO 100 Gel Electrophoresis System, Macintosh version,
1996
.11.
Baylor College of Medicine, inventors
. PE Applied Biosystems Corporation, assignee. AmpFlSTR Profiler PCR Amplification Kit
. US Patnet 5,364,759.12.
PE Applied Biosystems. AmpFlSTR Profiler PCR Amplification Kit User's Manual,
1997
.13.
PE Applied Biosystems. Performance Optimzed Polymer 4 (POP-4) US Patent 5,552,028.
14.
PE Applied Biosystems. Performance Optimized Polymer 6 (POP-6) US Patent 5,552,028.
15.
Comey
C T
, Koons
B W
, Presley
K W
, Smerick
J B
, Sobieralski
C A
, Stanley
D M
, et al DNA extraction strategies for amplified fragment polymorphism analysis
. J Forensic Sci
1994
;39
:1254
-69
.16.
Gentra Systems Inc.
Puregene DNA Isolation Kits
.17.
Perkin-Elmer Applied Biosystems QuantiBlot™ Human DNA Quantitation Kit User's Manual
, 1996
.18.
Baylor College of Medicine, inventors
. PE Applied Biosystems Corporation, assignee. AmplitypeR PM + DQA1 Amplification and Typing Kit
.19.
Perkin-Elmer Applied Biosystems AplitypeR PM + DQA1 Amplification and Typing Kit User's Manual
, 1994
.20.
Cosso
S
, Reynolds
R
. Validation of the AmpliFLP™ D1S80 PCR amplification kit for forensic casework analysis according to TWGDAM guidelines
. J Forensic Sci
1995
;40
:424
-34
.21.
Buel
, E
, Schwartz
M B
, LaFountain
M J
. Capillary electrophoresis STR analysis: comparison to gel-based systems
. J Forensic Sci
1998
;43
:164
-70
.22.
Walsh
P S
, Fildes
N J
, Reynolds
R
. Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA
. Nucleic Acids Res
1996
;24
:2807
-12
.23.
Giusti
A
, Baird
M
, Pasquale
S
, Balazs
I
, Glassberg
J
. Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm
. J Forensic Sci
1986
;31
:409
-17
.24.
Frank
W E
, Llewellyn
B E
. A time course study on STR profiles derived from human bone, muscle and bone marrow
. J Forensic Sci
1999
;44
:778
-82
.25.
Perkin-Elmer Applied Biosystems AmpFlSTR Blue PCR Amplification Kit User's Manual
, 1996
.
This content is only available via PDF.
All rights reserved. This material may not be reproduced or copied, in whole or in part, in any printed, mechanical, electronic, film, or other distribution and storage media, without the written consent of ASTM International.
You do not currently have access to this content.