Abstract

DNA samples from 101 unrelated individuals were extracted by Chelex procedure (1) and then quantified using QuantiBlot® Human DNA Quantitation Kit according to the manufacturer's instructions (2). DNA samples (1 ng) were amplified and typed by PowerPlex® 16 System (3). The electrophoresis was carried out on the ABI PRISM® 377 DNA Sequencer using GeneScan® Genotyper® and PowerTyper™ 16 Macro software. Data were analyzed using a program provided by R. Chakraborty (University of Texas School of Biomedical Sciences, Houston, Texas). Interclass correlations yielding p < 0.05 for pairwise comparisons: TH01/D7S820, TH01/D8S1179, D18S51/D5S818, D18S51/D7S820, PentaE/PentaD, and D5S818/D16S539.

Issue Section:
Research Papers
Keywords:
forensic science, DNA typing, population genetics, short tandem repeats, D3S1358, TH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTA D, vWA, D8S1179, TPOX, FGA, Argentina

References

1.
Walsh
 
PS
,
Metzger
 
DA
,
Higuchi
 
R
.
Chelex 100 as a medium for simple extraction of DNA for PCR-based from forensic material
.
Biotechniques
 0736-6205
1991
;
10
:
506
–
13
.
Google Scholar
PubMed
 
2.
Perkin Elmer. QuantiBlot® Human DNA Quantitation Kit
, Revised ed.,
05
1996
.
3.
PowerPlex® 16 System
, Technical Manual Part TMD012 Revised ed.,
03
2001
.
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