Abstract
Blood samples were obtained from selected and unrelated individuals. DNA was extracted with the standard Chelex® 100 (BioRad, Hercules, CA) extraction procedure (1); DNA samples were amplified in a DNA Gene Amp 9700 (Applied Biosystems, Foster City, CA) using 10 ng of template DNA. The amplified products were detected using the Abi Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).
Issue Section:
Research Papers
References
1.
Walsh
PS
, Metzger
DA
, Higuchi
R
. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material
. Biotecniques
0736-6205 1991
;10
:506
–13
.2.
DNA recommendations report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphism in STR (short tandem repeat) system
. Int J Legal Med
0937-9827 1994
;107
:159
–60
.
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