Abstract
Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/DXS6807.htm. The volume of PCR reaction for each locus was 37.5 L. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of female population genetics and forensic science were analyzed using POWERSTATS program (3).
Issue Section:
Research Papers
References
1.
Walsh
BS
, Petzger
DA
, Higuchi
R
. Chelex-100 as medium for simple extraction of DNA for PCR-based typing from forensic material
. Biotechniques
0736-6205 1991
;10
:506
–10
.2.
Allen
CR
, Graves
G
, Budowle
B
. Polymerase chain reaction amplification products separated on rehydratable polycrylamide gels and stained with silver
. Biotechniques
0736-6205 1990
;7
:736
–44
.
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