Abstract

Genipin, a hydrolytic product of geniposide extracted from gardenia fruit, was thoroughly studied as a potential fingerprint reagent, and optimal conditions for fingerprint development have been determined. Latent fingerprints on paper items that have been treated with a non-ink running formulation containing 0.17% of the reagent, showed up as both colored and fluorescent images. On brown wrapping paper and on papers with highly luminescent backgrounds, genipin developed more visible and clearer prints than did classical reagents such as ninhydrin or DFO. Another potential advantage of genipin is that it is totally harmless and an environmentally friendly reagent.

References

1.
Almog
J
,
Cohen
Y
,
Azoury
M
,
Hahn
TR
.
Genipin, a novel fingerprint reagent with colorimetric and fluorogenic activity
.
J Forensic Sci
 0022-1198
2004
;
49
(
2
):
255
7
.
2.
Kent
T
, editor.
Manual of fingerprint development techniques
. 2nd ed.
Sandridge
:
Home Office
,
1998
.
3.
Mexameter MX16
,
Operating Instructions
.
Koln, Germany
:
CK Electronic GmbH.
,
1999
.
4.
Avissar
Y
,
Sagiv
AE
,
Mandler
D
,
Almog
J
.
Identification of firearms handling by the [Fe(PDT)3]+2 complex: Chemical and time-dependent factors
.
Talanta
 0039-9140. In press.
5.
Cary Eclipse Fluorescence Spectrophotometer, Operating Instructions
.
St. Helens, Australia
:
Varian Australia Pty Ltd.
,
1999
.
6.
Polilight PL500, Operating Instructions
.
Victoria, Australia
:
Rofin Australia Pty. Ltd.
,
1998
.
7.
Video Spectral Comparator (VSC-1), Operating Instructions
.
United Kingdom
:
Foster and Freeman Ltd.
,
1995
.
8.
Petrovskaia
O
,
Taylor
BM
,
Hauze
DB
,
Carroll
PJ
,
Joullie
MM
.
Investigations of the reaction mechanisms of 1,2-indanediones with amino acids
.
J Org Chem
 0022-3263
2001
;
66
:7666.
9.
Lee
SW
,
Lim
JM
,
Bhoo
SH
,
Paik
YS
,
Hahn
TR
.
Colorimetric determination of amino acids using genipin from Gardenia jasminoides
.
Anal Chim Acta
 0003-2670
2003
;
480
:
267
74
.
10.
Moffat
KL
,
Marra
KG
.
Biodegradable poly(ethylene glycol) hydrogels crosslinked with genipin for tissue engineering applications
.
J Bio Materials Research, Applied Biomaterials
 1552-4973
2004
;
71B
(
1
):
181
7
.
11.
Friedman
M
.
Application of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences
.
J Agric Food Chem
 0021-8561
2004
;
52
:
385
406
and references therein.
12.
Piirila
P
,
Estlander
T
,
Hytonen
M
,
Keskinen
H
,
Tupasela
O
,
Tuppurainen
M
.
Rhinitis caused by ninhydrin develops into occupational asthma
.
Eur Respir J
 0903-1936
1997
;
10
(
8
):
1918
21
.
13.
Liu
BS
,
Yao
CH
,
Chen
YS
,
Hsu
HS
.
In vitro evaluation of degradation and cytotoxicity of a novel composite as a bone substitute
.
J Bio Materials Research
 0021-9304
2003
;
67A
(
4
):
1163
9
.
14.
Chang
WH
,
Chang
Y
,
Lai
PH
,
Sung
HW
.
A genipin-crosslinked gelatin membrane as wound-dressing material: in vitro and in vivo studies
.
J Biomaterials Sci—Polymer ed.
 0920-5063
2003
;
14
(
5
):
481
95
.
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