Understanding environmental factors relative to transfection protocols is key for improving genetic engineering outcomes. In the following work, the effects of temperature on a nonviral transfection procedure previously described as lance array nanoinjection are examined in context of molecular delivery of propidium iodide (PI), a cell membrane impermeable nucleic acid dye, to HeLa 229 cells. For treatment samples, variables include varying the temperature of the injection solution (3C and 23C) and the magnitude of the pulsed voltage used during lance insertion into the cells (+5 V and +7 V). Results indicate that PI is delivered at levels significantly higher for samples injected at 3C as opposed to 23C at four different postinjection intervals (t = 0, 3, 6, 9 mins; p-value ≤ 0.005), reaching a maximum value of 8.3 times the positive control for 3 C/7 V pulsed samples. Suggested in this work is that between 3 and 6 mins postinjection, a large number of induced pores from the injection event close. While residual levels of PI still continue to enter the treatment samples after 6 mins, it occurs at decreased levels, suggesting from a physiological perspective that many lance array nanoinjection (LAN) induced pores have closed, some are still present.
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November 2015
Research-Article
Transient Low-Temperature Effects on Propidium Iodide Uptake in Lance Array Nanoinjected HeLa Cells
John W. Sessions,
John W. Sessions
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: sessions.john84@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: sessions.john84@gmail.com
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Brad W. Hanks,
Brad W. Hanks
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: hanksbr90@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: hanksbr90@gmail.com
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Dallin L. Lindstrom,
Dallin L. Lindstrom
Department of Exercise Science,
Brigham Young University,
Provo, UT 84602
e-mail: dallin.lindstrom@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: dallin.lindstrom@gmail.com
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Sandra Hope,
Sandra Hope
Department of Microbiology and
Molecular Biology,
Brigham Young University,
Provo, UT 84602
e-mail: sandrahope2016@gmail.edu
Molecular Biology,
Brigham Young University,
Provo, UT 84602
e-mail: sandrahope2016@gmail.edu
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Brian D. Jensen
Brian D. Jensen
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: bdjensen@byu.edu
Brigham Young University,
Provo, UT 84602
e-mail: bdjensen@byu.edu
Search for other works by this author on:
John W. Sessions
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: sessions.john84@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: sessions.john84@gmail.com
Brad W. Hanks
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: hanksbr90@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: hanksbr90@gmail.com
Dallin L. Lindstrom
Department of Exercise Science,
Brigham Young University,
Provo, UT 84602
e-mail: dallin.lindstrom@gmail.com
Brigham Young University,
Provo, UT 84602
e-mail: dallin.lindstrom@gmail.com
Sandra Hope
Department of Microbiology and
Molecular Biology,
Brigham Young University,
Provo, UT 84602
e-mail: sandrahope2016@gmail.edu
Molecular Biology,
Brigham Young University,
Provo, UT 84602
e-mail: sandrahope2016@gmail.edu
Brian D. Jensen
Department of Mechanical Engineering,
Brigham Young University,
Provo, UT 84602
e-mail: bdjensen@byu.edu
Brigham Young University,
Provo, UT 84602
e-mail: bdjensen@byu.edu
1Corresponding author.
Manuscript received September 11, 2015; final manuscript received March 25, 2016; published online May 10, 2016. Assoc. Editor: Feng Xu.
J. Nanotechnol. Eng. Med. Nov 2015, 6(4): 041005 (9 pages)
Published Online: May 10, 2016
Article history
Received:
September 11, 2015
Revised:
March 25, 2016
Citation
Sessions, J. W., Hanks, B. W., Lindstrom, D. L., Hope, S., and Jensen, B. D. (May 10, 2016). "Transient Low-Temperature Effects on Propidium Iodide Uptake in Lance Array Nanoinjected HeLa Cells." ASME. J. Nanotechnol. Eng. Med. November 2015; 6(4): 041005. https://doi.org/10.1115/1.4033323
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